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p73 antibody img 259a  (Novus Biologicals)


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    Structured Review

    Novus Biologicals p73 antibody img 259a
    ( A ) Quantification: Proteins were extracted from C2C12 myoblasts treated with compounds: FCCP, Tunicamycin (Tun), Etoposide (Eto), menadione (Men). Western blot analysis revealed TAp63 expression. Bars correspond to means with SD (n = 3). *p<0.01. ( B ) C2C12 myoblasts were transfected with expression vectors encoding transcription factors involved in the ER or mitochondrial stress pathway (CHOP, ATF6, ATF4, XBP1s). RNA levels for TA isoforms of Trp63 , TA isoforms of <t>P73</t> and P53 were followed by RT-qPCR. Bars represent means (relative induction versus Ct) with standard deviation (n = 3).*p<0.01. DOI: http://dx.doi.org/10.7554/eLife.10528.016
    P73 Antibody Img 259a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p73+img+259a+imgenex/p73+Antibody/pmc04786414-262-37-40
    Average 90 stars, based on 26 article reviews
    p73 antibody img 259a - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Transcriptional activator TAp63 is upregulated in muscular atrophy during ALS and induces the pro-atrophic ubiquitin ligase Trim63"

    Article Title: Transcriptional activator TAp63 is upregulated in muscular atrophy during ALS and induces the pro-atrophic ubiquitin ligase Trim63

    Journal: eLife

    doi: 10.7554/eLife.10528

    ( A ) Quantification: Proteins were extracted from C2C12 myoblasts treated with compounds: FCCP, Tunicamycin (Tun), Etoposide (Eto), menadione (Men). Western blot analysis revealed TAp63 expression. Bars correspond to means with SD (n = 3). *p<0.01. ( B ) C2C12 myoblasts were transfected with expression vectors encoding transcription factors involved in the ER or mitochondrial stress pathway (CHOP, ATF6, ATF4, XBP1s). RNA levels for TA isoforms of Trp63 , TA isoforms of P73 and P53 were followed by RT-qPCR. Bars represent means (relative induction versus Ct) with standard deviation (n = 3).*p<0.01. DOI: http://dx.doi.org/10.7554/eLife.10528.016
    Figure Legend Snippet: ( A ) Quantification: Proteins were extracted from C2C12 myoblasts treated with compounds: FCCP, Tunicamycin (Tun), Etoposide (Eto), menadione (Men). Western blot analysis revealed TAp63 expression. Bars correspond to means with SD (n = 3). *p<0.01. ( B ) C2C12 myoblasts were transfected with expression vectors encoding transcription factors involved in the ER or mitochondrial stress pathway (CHOP, ATF6, ATF4, XBP1s). RNA levels for TA isoforms of Trp63 , TA isoforms of P73 and P53 were followed by RT-qPCR. Bars represent means (relative induction versus Ct) with standard deviation (n = 3).*p<0.01. DOI: http://dx.doi.org/10.7554/eLife.10528.016

    Techniques Used: Western Blot, Expressing, Transfection, Quantitative RT-PCR, Standard Deviation

    ( A ) mRNA levels of Trim63 in C2C12 cells following transfection with siRNA control and siRNA directed against p73, p53 and a mix of siRNA against P53, and the TA isoforms of Trp63 and P73 (siMIX). Bars represent means (relative induction versus Ct) with standard deviation (n = 3). *p<0.01. ( B ) mRNA level for TA isoforms of Trp63 , TA isoforms of P73 and P53 in C2C12 cells following transfection with siRNA control and siRNA directed against p63, p73, and p53. Bars represent means (relative induction versus Ct) with standard deviation (n = 3). *p<0.01. ( C, D ) Chromatin immunoprecipitation (ChIP) assay was performed on the Trim63 promoter using RT-qPCR on RE1/2 and RE4. p53 immunoprecipitation (C) was performed using p53 antibody IC12 (Cell Signalling), p73 immunoprecipitation was performed using p73 antibody IMG-259a (Imgenex). Bars correspond to means with SD (n = 3). *p<0.01 as calculated by a one-way ANOVA test followed by a Tukey post-test. DOI: http://dx.doi.org/10.7554/eLife.10528.019
    Figure Legend Snippet: ( A ) mRNA levels of Trim63 in C2C12 cells following transfection with siRNA control and siRNA directed against p73, p53 and a mix of siRNA against P53, and the TA isoforms of Trp63 and P73 (siMIX). Bars represent means (relative induction versus Ct) with standard deviation (n = 3). *p<0.01. ( B ) mRNA level for TA isoforms of Trp63 , TA isoforms of P73 and P53 in C2C12 cells following transfection with siRNA control and siRNA directed against p63, p73, and p53. Bars represent means (relative induction versus Ct) with standard deviation (n = 3). *p<0.01. ( C, D ) Chromatin immunoprecipitation (ChIP) assay was performed on the Trim63 promoter using RT-qPCR on RE1/2 and RE4. p53 immunoprecipitation (C) was performed using p53 antibody IC12 (Cell Signalling), p73 immunoprecipitation was performed using p73 antibody IMG-259a (Imgenex). Bars correspond to means with SD (n = 3). *p<0.01 as calculated by a one-way ANOVA test followed by a Tukey post-test. DOI: http://dx.doi.org/10.7554/eLife.10528.019

    Techniques Used: Transfection, Control, Standard Deviation, Chromatin Immunoprecipitation, Quantitative RT-PCR, Immunoprecipitation

    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo.
    Article Snippet: .. TAp63 andDNp63 ChIP analysis was performed using a pan-p63 antibody (4A4, Santa Cruz) as described previously and the TAp73 ChIP was performed using a TAp73 antibody (ab14430, Abcam) andDNp73 ChIP was performed using a p73 antibody (IMG 259A, Imgenex). ..

    Article Title: Transcriptional activator TAp63 is upregulated in muscular atrophy during ALS and induces the pro-atrophic ubiquitin ligase Trim63
    Article Snippet: .. *p<0.01. ( C, D ) Chromatin immunoprecipitation (ChIP) assay was performed on the Trim63 promoter using RT-qPCR on RE1/2 and RE4. p53 immunoprecipitation (C) was performed using p53 antibody IC12 (Cell Signalling), p73 immunoprecipitation was performed using p73 antibody IMG-259a (Imgenex). ..

    Article Title: IAPP driven metabolic reprogramming induces regression of p53 - deficient tumours in vivo
    Article Snippet: .. TAp63 and ΔNp63 ChIP analysis was performed using a pan-p63 antibody (4A4, Santa Cruz) as described previously and the TAp73 ChIP was performed using a TAp73 antibody (ab14430, Abcam) and ΔNp73 ChIP was performed using a p73 antibody (IMG 259A, Imgenex). ..

    other:

    Article Title: CK2 Phosphorylates and Inhibits TAp73 Tumor Suppressor Function to Promote Expression of Cancer Stem Cell Genes and Phenotype in Head and Neck Cancer
    Article Snippet: Western blot analysis and co-immunoprecipitation was performed as previously with antibodies indicated, CK2α (Santa Cruz, sc-6479), CK2α′ (Santa Cruz, sc-6481), Nanog (Cell Signaling, 4903), Oct4 (Cell Signaling, 4286), Sox2 (Cell Signaling, 2748), beta-actin (Cell Signaling, 4967), TAp73 (IMGENEX,IMG-246), p73 (IMGENEX,IMG-259A), Oct-1 (Santa Cruz, sc-53830), Flag antibody(Sigma, M2), PUMA (Cell Signaling, 4976).

    Incubation:

    Article Title: p63 and p73 Transcriptionally Regulate Genes Involved in DNA Repair
    Article Snippet: .. Slides were incubated with primary antibodies for p63 (4A4, Santa Cruz), p73 (IMG-259A, Imgenex), Rad51 (clone 51RAD01, Neomarkers), or BRCA2 (clone H-300), Santa Cruz). at a dilution of 1∶100 for 18 hours at 4 deg C. Detection was performed using the Vectastain kit (Vector Labs) followed by the VIP kit or DAB kit (Vector Labs) and counterstained with methyl green (Vector Labs). ..

    Quantitative RT-PCR:

    Article Title: Transcriptional activator TAp63 is upregulated in muscular atrophy during ALS and induces the pro-atrophic ubiquitin ligase Trim63
    Article Snippet: .. *p<0.01. ( C, D ) Chromatin immunoprecipitation (ChIP) assay was performed on the Trim63 promoter using RT-qPCR on RE1/2 and RE4. p53 immunoprecipitation (C) was performed using p53 antibody IC12 (Cell Signalling), p73 immunoprecipitation was performed using p73 antibody IMG-259a (Imgenex). ..

    Immunoprecipitation:

    Article Title: Transcriptional activator TAp63 is upregulated in muscular atrophy during ALS and induces the pro-atrophic ubiquitin ligase Trim63
    Article Snippet: .. *p<0.01. ( C, D ) Chromatin immunoprecipitation (ChIP) assay was performed on the Trim63 promoter using RT-qPCR on RE1/2 and RE4. p53 immunoprecipitation (C) was performed using p53 antibody IC12 (Cell Signalling), p73 immunoprecipitation was performed using p73 antibody IMG-259a (Imgenex). ..

    Article Title: p63 and p73 Transcriptionally Regulate Genes Involved in DNA Repair
    Article Snippet: .. Cellular proteins were crosslinked to chromatin with 1% formaldehyde. p53-DNA, p63-DNA or p73-DNA complexes were immunoprecipitated using the following antibodies: pan-p63 (4A4, Santa Cruz), pan-p73 (IMG-259a, Imgenex) or p53 (Ab-3, Oncogene Research Products). .. Total RNA was extracted from the E1A MEFs of the genotypes described above using the RNeasy Midi and Rnase-free Dnase kits (Qiagen).

    Concentration Assay:

    Article Title: TAp73 enhances the pentose phosphate pathway and supports cell proliferation
    Article Snippet: .. Antibodies against the following proteins/epitopes were used in this study with the company, catalog number, and dilution or concentration indicated: GFP (Clontech Laboratories, Mountain View, CA; 632381; 1:4,000), actin (Sigma-Aldrich, St Louis, MO; A2066; 1:4,000), Flag (Sigma, F3165, 1:4,000), p53 (DO-1) (Santa Cruz Biotechnology, Santa Cruz, CA; sc-126 HRP; 1:1,000), p73 (Imgenex Corp, San Diego, CA; IMG-259A; 1:1,000) (Bethyl Laboratories, Montgomery, TX; A300-126A; 1:1,000) (Sigma, SAB4300354, 1:1,000), mouse p73 (Santa Cruz, sc-7238, 1:500) (Cell Signaling Technology, Danvers, MA; 4300354; 1:1,000), G6PD (Sigma, HPA000834, 1:3,000), p21 (Cell Signaling, 2947, 1:1,000), and fluorescein isothiocyanate (FITC)-labeled anti-BrdU antibody (BD Bioscience Pharmingen; 556028; 10 μM). ..



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